Fig. 6. Genetic interactions between the SIN and flp 1. (A) Cells were
grown in YE medium at 25°C and shifted to 36°C for 5 hours, fixed and
stained with DAPI and Calcofluor. (B) Cells were grown at 29°C, then
shifted for 8 hours to 25°C or for 5 hours to 36°C, before fixation
and staining with DAPI and Calcofluor. (C,D) flp 1::kanMX6 leu 1-32
and leu 1-32 (WT) strains were transformed to leucine prototrophy
with pREP3-spg 1 (C) or pREP41-byr4 (D). Expression of the
genes was induced for 18 hours at 25°C, then cells were fixed and stained
with DAPI and Calcofluor.
