Fig. 9. Mitotic control mutants have altered sensitivity to increased expression of
flp1+. A single, nmt1-promoter-controlled copy of
flp1+ was introduced into the leu1 locus and
crossed into the indicated strain backgrounds. Expression of
flp1+ was induced and cell number was determined at
intervals thereafter. Cells were grown at 25°C. (A) The effect of
increased flp1+ expression in wee1-6 and
cdc25::ura4+ wee1-50. Induced images were taken
26 hours (approximately 6 generations) after induction. (B) Cell number
increase after induction of flp1+. Cell numbers are
expressed in arbitrary units. Cell numbers at 16 hours were approximately
106 ml-1. (C,D) Induction of flp1+
in dominant activated alleles of cdc2.
