Fig. 3. scar- cells contain mostly small acidic vesicles. Phase
contrast (A,C) and fluorescent (B,D) microscopic images of control (A,B) and
scar- cells (C,D). (A-D) Cells were incubated with
FITC-dextran for 1 hour, washed and spotted onto coverslips prior to
examination. In control cells (A,B), vesicles of many different sizes were
present, including large post-lysosomal and macropinosomal vesicles (arrows).
By contrast, scar- cells contained primarily smaller
vesicles. (E,F) scar- cells were incubated with
RITC-dextran for 1 hour, washed and further incubated with Lysosensor Green
for 10 minutes. After spotting cells on coverslips, they were examined using
the red channel (E) or the green channel (F) of the fluorescence microscope.
Most of the RITC-dextran-positive vesicles (E) also stained with Lysosensor
Green (F), indicating that most of the vesicles in the
scar- cells were acidic. Bar, 5 µm.
