Fig. 7. Cells with the
pI-/II-/scar-
triple mutation contain mainly small acidic endosomes. To examine the
morphology of the endo-lysosomal system of
pI-/II- and
pI-/II-/scar-
mutants, cells were incubated with FITC-dextran for 1 hour, spotted on
coverslips and prepared for phase contrast (A,C,E) or fluorescence (B,D,F)
microscopy. Control cells (A,B) contained vesicles of many different sizes,
representing pinosomes, macropinosomes, lysosomes and post-lysosomes. By
contrast, pI-/II- and
pI-/II-/scar-
cells (C-F) contained only smaller vesicles that are presumed to be lysosomes,
because they stained with an acidic fluorophore (data not shown). Bar, 5
µm.
