Fig. 12. Fluorescence recovery after photobleaching (FRAP) analysis showing the
direction of movement of FM4-64-stained material around the pollen tube. (A,B)
Movement from the apex down the centre of the cell to more subapical regions.
(A) Time sequence of confocal fluorescence images; time relative to bleaching
is in seconds. The original area of bleaching is shown as the boxed area. (B)
Pixel intensity plots along a central transect through the images in A. The
original bleached zone is indicated as the boxed area. The relative position
of the original bleached zone to the apex is shown as the dashed boxed area;
graphs have been aligned from the cell apex. (C,D) Movement from subapical
peripheral regions into the apex. (C) Time sequence of confocal fluorescence
images; the first image indicates the lateral bleaching areas (box a). (D)
Graphs of fluorescence intensity against time for the areas indicated a-c. The
relative position of the sample areas to the apex, shown in the first image of
(C), was maintained throughout. The grey shaded area indicates time of
bleaching. Bars, 15 µm.
