Fig. 13. Quantitative analysis of the relationship between FM4-64 staining and
oscillatory growth of a L. longiflorum pollen tube. (A) Growth rate
for a pollen tube derived from confocal images of FM4-64 fluorescence. (B-D)
Corresponding plots of average pixel value against time for the regions A-C
defined in diagram (E). Heavy dashed lines show peak growth rate; grey boxed
areas show the times when the apical 3-5 µm region is most distinctly
defined from the more subapical FM4-64 staining (see Results and F). (E)
Sample areas A-C: apical 1-3 µm, 5 µm and 10 µm subapically,
respectively. (F) Graphs of pixel values along a central transect of the
pollen tube are shown (as in Fig.
1) for images covering the growth period outlined by the
cross-hatched area in A. The maximum and minimum rate growth phases of the
period are indicated.
