Fig. 8. FM4-64-washout time course from a growing pollen tube of L.
longiflorum preloaded with dye for 3 hours. Washout was achieved by
continuous perfusion with dye-free medium. (A) Selected images showing the
pattern of decline in fluorescence; times relative to the start of perfusion
are given in minutes. (B) Plots of normalised fluorescence intensity within
four regions, defined in A by the areas a-d: (a) extreme apex, (b) subapical
region, (c) plasma membrane (plotted as c minus d) and (d) periphery. Bar, 15
µm.
