Fig. 5. Effect of anti-CD95 stimulation on the intracellular distribution of CD95
and FAP-1 in Panc89 cells. Under control conditions (A,G,K) the majority of
FAP-1 immunoreactivity (depicted in red) is localised in the Golgi area (A,
arrowhead). Some additional peripheral vesicular staining is also seen. After
stimulation of Panc89 cells with agonistic APO1-3 antibody (2 µg/ml) for 5
minutes (B,H) or 15 minutes (C,I) this staining pattern remains principally
unaltered (B,C, arrowheads), however, immunoreactivity is strongly enhanced
after 15 minutes. CD95 immunoreactivity (depicted in green) is found at the
plasma membrane but is predominantly intracellular under control conditions
(D,G,K). Within 5 minutes of APO1-3 incubation of Panc89 cells (E,H),
immunoreactivity for CD95 is enhanced, and pronounced plasma membrane staining
is found, especially at sites of cell-cell-contact (see arrows in E). After 15
minutes of CD95 stimulation, strong immunoreactivity for CD95 is spread all
over the cell and is concentrated in the Golgi area (arrowhead in F), thus
showing strong colocalization with FAP-1 (yellow colouring, arrowhead in I)
while plasma membrane staining for CD95 is reduced. In a parallel experiment
Panc89 cells were stimulated with 100 ng/ml TRAIL (control, K) for 5 minutes
(L) and 15 minutes (M). No significant changes were observed in CD95 (green)
and FAP-1 (red) staining patterns.
