Fig. 4. Differential gene expression during capillary morphogenesis as determined
by western blot analysis and immunohistochemistry. EC-collagen gel extracts or
conditioned medium were prepared or collected at the indicated time points.
Samples were run on SDS-PAGE and, after blotting, were probed with antibodies
directed to various proteins. Cell extracts were probed with antibodies
directed to collagen type IV, 
2-macroglobulin and Id-1.
Conditioned medium samples were probed with antibodies directed to collagen
type IV or laminin. The arrows in the collagen type IV blots represent the
1 and 2 chains of collagen type IV. Arrows indicate the position
of molecular weight markers on the blots. In B, a 48 hour culture was stained
with antibodies to collagen type IV under non-permeabilizing conditions. No
staining was observed without the addition of the primary anti-collagen type
IV antibody. Bar, 50 µm.
