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Fig. 5. Differential expression of extracellular matrix and integrin genes during
capillary morphogenesis. RT-PCR was performed to assess the mRNA expression
patterns of various extracellular matrix proteins (A) and integrin subunits
(B) over a 48 hour time course. Total RNA was prepared from EC cultures at 0,
8, 24 and 48 hours of culture. The RNA was reverse transcribed for RT-PCR and
PCR was performed using gene specific primer sets.
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