Fig. 6. Interference with collagen type IV synthesis and secretion blocks capillary
morphogenesis in 3D collagen matrices. EC cultures were treated with or
without ethyl 3,4-dihydroxybenzoate (edb), an inhibitor of prolyl hydoxylase.
Cultures were fixed at varying times, stained, quantitated for lumen formation
(B) and photographed (A). Bar, 50 µm (A). Cell extract samples (from
cultures with or without edb at 300 µM) and at different times of culture
were collected and run on 7% SDS-PAGE gels (C). Lanes 1 and 2, 0 hours; lanes
3,4, 8 hours; lanes 5,6, 24 hours; lanes 7,8, 48 hours; lanes 9,10, 72 hours.
Proteins were blotted to PVDF membranes and were probed with antibodies to
collagen type IV. Arrows indicate the position of the 
1 and 2
chains of collagen type IV.
