Fig. 2. Expression of integrin 
Vß3 induced by RANKL-ODF treatment. (A)
Chicken peripheral blood-derived macrophages were cultured on glass coverslips
for four days in the presence (right panels) or absence (left panels) of
RANKL-ODF (see Materials and Methods). Cells were fixed, permeabilized and
stained with the monoclonal antibody 23C6 (green) and rhodamine-phalloidin
(red). Confocal images were acquired using identical channel settings for
Vß3 detection and were equally processed in Adobe Photoshop to
ensure comparable detection of Vß3 expression. Upper panels,
23C6-staining only; lower panels, 23C6 + rhodamine phalloidin staining. (B)
Human osteoclast obtained after a nine day treatment of peripheral blood
monocytes with RANKL-ODF. Staining was performed as in A and revealed by
confocal microscopy. Upper panel, rhodamine phalloidin-staining (red); lower
panel, 23C6- (green) + rhodamine phalloidin-staining. Note the nearly complete
absence of integrin Vß3 on cells that were not treated with (A,
left panels) or that did not visually respond to (B) the osteoclastogenic
factor RANKL-ODF. Scale bar: 20 µm).
