Fig. 5. Distribution of paxillin, Pyk2, integrin 
Vß3 and cortactin in
the podosome zones of chicken and human osteoclast-like cells. Confocal
immunofluorescence images of cells double-stained with rhodamine phalloidin
(red) and with antibodies for paxillin, Pyk2, Vß3 or cortactin
(green in colored images or white in grayscale images) are shown. Right-hand
panels are high-power views, which were also scanned in the z-axis at
positions indicated by the double-arrows. These z-axis scans are
shown on top of these panels with the extracellular substrate-facing cell side
oriented towards the bottom of the images. Note the co-distribution of
paxillin, Pyk2 and Vß3 around and between, but not within, the
F-actin core structures of podosomes. By contrast, cortactin shows an inverse
distribution, i.e. colocalization with F-actin in xy-scans, but
appears also orientated towards the basal podosome side, as indicated by the
separation of red (F-actin) and green (cortactin) channels in the inset
scanned in the z-axis.
