Fig. 7. Distribution pattern of Grk protein in egg chambers from Tat transgenic
females. Confocal images of indirect immunofluorescent staining of egg
chambers with anti-Grk antibody using a Cy3-conjugated anti-rat secondary
antibody. The staining of the Grk protein is in green. F-actin is visualized
with rhodamine-conjugated phalloidin in red; regions where labels overlap are
yellow. All egg chambers are at stage 10 of oogenesis. (A) Egg chamber from a
female not expressing Tat. The Grk protein is normally localized to the
anterior-dorsal cortex of oocyte. (B,C) Egg chambers from females after Tat
induction. Grk is localized around the entire circumference (B) or at the
anterior margin (C).
