Fig. 1. Wild-type and mutant VSV-G epitope-tagged QNR-71 constructs used in the
study. Schematic representation of the QNR-71 gene product showing the
predicted signal sequence (SS), lumenal, transmembrane (TMD) and cytoplasmic
domains. The number of amino acids in each domain is indicated. The potential
sorting signals in the predicted cytoplasmic domain are shown in bold. The
VSV-G epitope is located at the N terminus of the gene product immediately
downstream of the signal sequence. The gpI-QNR-71 chimeric protein is composed
of the lumenal and transmembrane domains of the VZV envelope glycoprotein gpI
fused to a part of the QNR-71 tail (amino acids 537 to 556) containing a
di-leucine motif.
