Fig. 6. AP-3 dependent transport of QNR-71. HeLa cells were infected with a
recombinant T7 RNA polymerase vaccinia virus and transfected with a plasmid
encoding VSV-G-tagged QNR-71. After 3 hours of expression, the cells were
fixed and labeled with a polyclonal (a) or monoclonal (c) anti VSV-G antibody
and the 100/3 monoclonal anti-
-adaptin (b) or the polyclonal
anti-
-adaptin (d) antibody, to detect AP-1 and AP-3 complexes,
respectively. QNR-71 was detected using FITC-conjugated secondary antibodies.
- and -adaptin were decorated with Texas Red-coupled secondary
antibodies. Under these experimental conditions, the bulk of the expressed
protein is detected in the perinuclear area and has not yet reached the
endosomes. Non-transfected cells are indicated with an asterisk.
