Fig. 9. AP-3 dependent transport of QNR-71. HeLa cells transfected with the
wild-type VSV-G-QNR-71 construct were grown on glass coverslips. They were
either left untreated (a,d) or treated with sense (b,e) or antisense (c,f)
oligonucleotides. After 48 hours, antibodies directed against LampI (d-f) and
VSV-G (a-c) were added to cell culture medium and allowed to be internalized
for 4 hours at 37°C. After extensive washes, the cells were fixed and the
internalized antibodies were detected using fluorescently labeled secondary
antibodies. Asterisks indicate untransfected cells.
