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Live observation of fission yeast meiosis in recombination-deficient mutants: a study on achiasmate chromosome segregation

Monika Molnar1, Jürg Bähler2, Jürg Kohli1 and Yasushi Hiraoka3

1Institute of Cell Biology, University of Bern, CH-3012 Bern, Switzerland
2The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
3Cell Biology Group and CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, Kobe 651-2492, Japan




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Movie 1A

Meiotic prophase nuclear movements in a wild-type cell of S. pombe. The nucleus moves back and forth between the cell poles. The nucleus was stained with Hoechst 33342 (see Fig. 1A).





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Movie 1B

Aberrant nuclear movements observed in meiotic prophase in a rec8 mutant (see Fig. 1B).





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Movie 1C

Aberrant nuclear movements observed in meiotic prophase in a rec7 mutant (see Fig. 1C).





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Movie 2A

Nuclear dynamics during the first meiotic division in a rec8 mutant (see Fig. 2A).





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Movie 2C

A chromosome wandering between the cell poles during the first meiotic division in a rec7 mutant (see Fig. 2C).





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Movie 4

Separation of homologous chromosomes in the first meiotic division in wild-type. A locus near the centromere 1 was marked by lacI-GFP. GFP signals arising from homologous chromosomes move to the opposite poles (see Fig. 4).





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Movie 5A

Nondisjunction of homologous chromosomes in the first meiotic division in rec7. GFP signals arising from homologous chromosomes eventually moved to the same pole, resulting in nondisjunction (see Fig. 5A).





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Movie 5B

Chromosome re-orientation in the first meiotic division in rec7. The chromosome that moved to the same pole as its homolog subsequently re-oriented to the other pole (see Fig. 5B).





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Movie 6

Precocious separation of sister chromatids in the first meiotic division in rec8. Only one of the homologous chromosomes was marked in this example, thus the separating signals arose from sister chromatids (see Fig. 6).




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