Fig. 3. Quantitative analysis of the distribution of cytochrome c and mitochondrial
diameter change. (A,B) Magnified images of Mitotracker before (A) and after
(B) mitochondria swelled. (C) The diameter of a mitochondrion was taken as the
half width of its pixel profile based on a line-scan measurement of the
Mitotracker image. (D) The distribution profiles of cytoc-GFP fluorescence
intensity in a region containing only cytoplasm (left), or in a region
containing both cytoplasm and mitochondria (right). The arrow marks the
threshold that separates the fluorescence signal contributed by cytoc-GFP
located in the mitochondria from that of the cytosolic cytoc-GFP.
