Fig. 3. Genomic structure of LTBP-4 around the 3rd 8-Cys repeat. (A)
PAC/LTBP-4 clone containing genomic sequences from LTBP-4 was used as a
template in PCR amplification using LTBP-4.2 and `3rd 8-Cys' primers. The `3rd
8-Cys' primers amplify a region that is absent from
LTBP-4
8-Cys3rd (for primer sequences see
Table 1). The migration of
4.5 kb and 650 bp PCR products in an agarose gel is shown. The
molecular weight markers (nt) are shown on the left. (B) Schematic
representation of LTBP-4 as well as the exon-intron structure around the
3rd 8-Cys repeat. The sequences obtained from splice-site junctions
of introns A, B and C are shown.
