Fig. 5. RNase protection assay from tissue-derived mRNA. RNase protection assays
were performed with 0.5 µg of polyA+ RNA derived from different
tissues as illustrated. The probe, `RPA-2', was radioactively labeled and
incubated with or without tissue mRNA as in
Fig. 4A. Relative values of
LTBP-4 mRNAs (see text for details) compared with LTBP-4 levels in the heart
(set as 100%). Phosphoimager quantification of two separate experiments is
presented with error bars. (Inset) RNase protection assay of heart and lung
mRNAs. The protected fragments corresponding to LTBP-4 containing the
3rd 8-Cys repeat, LTBP-4
8-Cys3rd as well as
LTBP-4EGF-13 are indicated on the right. The mobilities of the
molecular weight markers (nt) are shown on the left of the inset.
