Fig. 1. Defective phenotypes of the wat1 mutant and gene disruption. (A)
Diploidisation. Wild-type (top) pop1-364 (middle) or
wat1-5235 cells (bottom) were grown in rich medium at 27°C and
processed for flow cytometry (FACS). The left panels show the DNA content of
individual cells on the x-axis in a logarithmic scale and frequency
at the y-axis, while the right panels show forward scattering on the
x-axis and a DNA content on the y-axis. (B) Temperature and
cold sensitivity. Wild type (left) or wat1-5235 cells (right) were
streaked on rich plates and incubated at 19°C, 27°C or 36°C. (C)
Tetrad analysis. Two sets of tetrads, derived from heterozygous diploids for
the wat1+ gene (I030,
Table 1) and grown at 27°C
are shown. (D) Diploidisation of the wat1 disruptant.
wat1-deleted mutants were streaked on rich medium containing phloxine
B and incubated at 27°C for 3 days. White colonies (arrows) show haploid
cells, while dark red colonies (arrowheads) are diploid cells (confirmed by
FACS).
