Fig. 4. The wat1 mutant shows reduced 
-tubulin levels. (A)
Reduction of -tubulin levels but not other proteins. Protein extracts
were prepared from exponentially growing wild-type or wat1-5235
strain, run on SDS-PAGE and immunoblotting was performed with various
antibodies shown. (B) Decrease of -tubulin levels upon upwards
temperature shift. Wild-type (lanes 1, 4 and 7), wat1-5235 (lanes 2,
5 and 8) or wat1-deleted cells (lanes 3, 6 and 9) were grown at
27°C (lanes 1 to 3) and shifted up to 36°C. After 2 (lanes 4 to 6) and
4 hours (lanes 7 to 9), protein extracts were prepared and immunoblotting was
performed with anti--tubulin (top) or anti-Cdc2 antibody (bottom).
