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Fig. 2. Wild-type Sbf1 lacks oncogenic effects in NIH 3T3 cells. (A) Soft agar
assays were performed using NIH 3T3 cell lines expressing the indicated
constructs. Data are the average of three experiments performed in triplicate.
(B) Representative colony growth in soft agar is shown for the cell lines
tested. (C) Western blot analysis using an anti-Sbf1 monoclonal antibody
demonstrates comparable expression of wild-type Sbf1 and Sbf1
N750 in
stably transfected NIH 3T3 cells.
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