Fig. 5. Forced expression of wild-type Sbf1 inhibits proliferation and induces
morphological changes in NIH 3T3 cells. (A) Growth curves show the increase in
cell numbers for NIH 3T3 cells expressing wild-type or mutant Sbf1 constructs.
Data are shown for a representative experiment. (B) Proliferation rates of
stably transfected NIH 3T3 cells were determined by BrdU incorporation. Data
represent the average and standard deviations from three experiments performed
in duplicate. (C) Cytoskeletal architecture of NIH 3T3 cell stably transduced
with wild-type Sbf1 (left) or vector (right). Green fluorescence corresponds
to tubulin staining, which was revealed using primary anti-tubulin and
secondary fluorescein isothiocyanate-conjugated antibodies. DAPI staining is
shown in blue.
