Fig. 6. Wild-type Sbf1 and myotubularin are cytoplasmic proteins. (A) Western blot
analyses of Sbf1, G6PD and c-Myb performed on nuclear (N) and cytoplasmic (C)
fractions prepared from Raji cells show Sbf1 predominantly in the cyoplasmic
fraction. (B) Immunohistochemical analysis of Sbf1 in tissue sections using an
anti-Sbf1 monoclonal antibody shows specific cytoplasmic staining for Sbf1 in
the cerebellum from wild-type (left) but not Sbf1-/-
(right) mice. (C) NIH 3T3 cells were examined by immunofluorescence 48 hours
after co-transfection of constructs expressing FLAG-tagged Sbf1 or MTM1. Green
fluorescence corresponds to FLAG-tagged Sbf1 or MTM1 staining, which was
revealed using primary anti-FLAG and secondary fluorescein
isothiocyanate-conjugated antibodies.
