Fig. 3. pHi during the cell cycle. Phluorin-expressing wild-type or
cdc2-1w cells were grown to early logarithmic phase in EMM pH 5.5 and
the pHi and cell length measured for each individual cell. Top:
scatter plots of internal pH as a function of cell length. Open diamonds
indicate binucleate cells, whereas closed diamonds indicate uni-nucleates. The
linear line of best fit is shown in red. Approximate cell-cycle phases are
indicated. Bottom: cells were classified into arbitrary 1 µm size classes
and the mean internal pH of the created classes calculated. Error bars
indicate s.d.
