Fig. 1. Hepatocytes showed 8-hydroxyguanine DNA adducts following partial
hepatectomy. The liver was immunostained to demonstrate oxidative DNA injury
as described in Materials and Methods. (A) No nuclear signal was observed in
negative controls when the primary antibody was omitted. (B) Liver removed in
a partial hepatectomy showing staining of occasional cell nuclei (arrows). The
immunostaining is present in hepatocytes, as well as nonparenchymal cells. (C)
Liver from the same animal as in B 5 days after two-thirds partial hepatectomy
showing extensive and more intense nuclear staining of hepatocytes (arrows).
Nonparenchymal cells also exhibit increased DNA injury following partial
hepatectomy. (D) Higher magnification view of the liver in C showing stippled
staining of nuclear DNA in hepatocytes. Note presence of polyploid cells with
megalonuclei (arrow). The inset shows an apoptotic cell with nuclear fragments
containing stained DNA (inset). Oxidative DNA injury was seen in 40-50% of the
hepatocytes after partial hepatectomy. p, portal area.
