Fig. 2. Evidence for oxidative injury in hepatocytes from rats with or without
partial hepatectomy. The cells were cultured for 5 days followed by
measurement of catalase activity (A), glutathione content (B) and lipid
peroxidation (C). Cells from partially hepatectomized livers showed depletion
of catalaste and glutathione from the beginning, whereas these activities
declined significantly with time in cells isolated from the unperturbed liver.
Radiation treatment induced dose-dependent changes in catalase and glutathione
activities, which declined progressively in cultured cells. Catalase activity
eventually became undetectable in cells from partially hepatectomized livers
following radiation. These findings suggest greater lipid peroxidation in
cells from partially hepatectomized livers following radiation. All
measurements were taken in triplicate and data were reproduced in two
independent experiments.
