Fig. 4. Flow cytometric evidence for induction of polyploidy in hepatocytes. Cells
were isolated from the unperturbed normal rat liver and cultured for 5 days
with various treatments as indicated. (A) Flow cytometric profiles from
control untreated cells, and cells treated with TGF
, norepinephrine
(NE), vasopressin (VP) and 30 Gy radiation. DNA content is on the x axis with
diploid (2C), tetraploid (4C) and octaploid (8C) peaks as shown and the y axis
is the number of nuclei analyzed. (B) Analysis of ploidy distribution data
from studies shown in A. (C) Laser scanning cytometric analysis of sorted
propidium iodide-stained nuclei from diploid, tetraploid and octaploid DNA
peaks with columns 1 to 3 showing three representative examples each of sorted
nuclei with progressive nuclear enlargement.
