Fig. 5. Confocal imaging of meiotic chromosomes and spindles from B6 and Rb/+
spermatocytes (ß-tubulin in red, phospho-histone H3 in green). (A) A MI
spermatocyte from a control B6 mouse in which all chromosomes are found to be
properly aligned on the metaphase plate. (B) A MI spermatocyte from a Rb/+
mouse, illustrating unaligned chromosomes (arrowheads). (C) A MI spermatocyte
from a Rb/+ mouse displaying a misaligned chromosome (arrowhead) and an
abnormal spindle, in which one pole is undeveloped (arrow). (D) Rb/+ MII
spermatocytes depicting chromosomes lagging behind the spindle poles
(arrowheads). Scale bar: 10 µm.
