Fig. 1. In vitro migration assay. MCF10A cells were plated inside a glass ring and
were allowed to migrate as an outgrowth after the ring removal. (A) Sequential
video images of a migratory culture of MCF10A cells, taken immediately, 24, 48
or 72 hours after the removal of the ring and showing the expansion of the
outgrowth. (B) Images of nuclei stained with Hoechst dye and visualized under
epifluorescence illumination (a, at the edge of the outgrowth; c, distant from
the outgrowth periphery). Trajectories of 20 randomly selected nuclei
(indicated with a white dots in a and c) were quantified in each area (b, at
the edge of the outgrowth; d, distant from the outgrowth periphery). Scale
bars: 2.35 mm in A; 80 µm in B.
