Fig. 3. Specific overexpression and subcellular organization of MT1-MMP in
migratory MCF10A cells at the periphery of the outgrowth. (A) In situ
hybridization on a migratory culture of MCF10A cells performed with a MT1-MMP
antisense probe 72 hours after the removal of the ring. (B) MT1-MMP
immunolabelling on a migratory culture of MCF10A cells. MT1-MMP labelling is
in red and DAPI staining is in blue. (C) MT1-MMP immunolabelling (in red) on a
migratory culture of MCF10A cells (a). Phase-contrast microscopy analysis of
the corresponding area (b). (D) Confocal microscopy analyses of MT1-MMP in
migratory MCF10A cells 72 hours after the removal of the ring. Twenty-four
successive optical sections were taken from the apical (section 2) to the
basal (section 22) surface of the cells (sections 2, 12, 15, 16, 20 and 22 are
shown). MT1-MMP labelling was mainly found in the lamellipodia at the leading
edge of migratory cells (arrows) as well as at the basal surface of the cells
in contact with the substrate (section 16 and 20). Scale bars: 60 µm in
A,B; 20µm in C; 10 µm in D.
