Fig. 6. Ln-5 
2 chain degradation in migratory cultures of MCF10A cells and
cleavage of Ln-5 2 by MT1-MMP. (A) Western blot analyses of the Ln-5
2 subunit in migratory cultures of MCF10A cells incubated in complete
growth medium (MCF10A/FCS) 72 hours after the removal of the ring, in purified
Ln-5 from SCC25 cells used as a control and in purified Ln-5 incubated with
increasing amounts of the catalytic domain of human MT1-MMP (100 ng and 500
ng). (B) Western blot analyses of the Ln-5 2 subunit 72 hours after the
removal of the ring in migratory cultures of MCF10A cells incubated in
complete growth medium (MCF10A/FCS), in EGF-induced migratory cultures
(MCF10A/EGF) and in stationary cultures incubated in EGF/FCS-free medium
(Control). (C) Western blot analyses of MT1-MMP in migratory cultures of
MCF10A cells incubated in complete growth medium (MCF10A/FCS), in EGF-induced
migratory cultures (MCF10A/EGF) and in stationary cultures incubated in
EGF/FCS-free medium (Control). HT1080 cells are used as a control showing the
three main forms of MT1-MMP at 63, 60 and 43 kDa.
