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Fig. 2. Alterations of cell morphology and focal adhesions in Swiss 3T3 cells
expressing PYK2. Swiss 3T3 fibroblasts were microinjected with
plasmids encoding Myc-tagged PYK2 (A-C,G-I) or FAK (D-F,J-L). Cells were fixed
for 2-4 hours after injection and immunostained with anti-PYK2 (polyclonal,
against PYK2 amino acids 587-988) (A,G) and anti-vinculin (monoclonal) (B,E)
or anti-FAK (monoclonal) (D,J) antibodies, or with phalloidin (H,K); C, F, I
and L are overlay pictures. Bar, 50 µm.
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