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Fig. 3. Alterations of focal adhesions in 3Y1, 10T1/2 and MDCK cells expressing PYK2. 3Y1 (A,B), 10T1/2 (C,D) and MDCK (E,F) cells were transiently transfected with plasmid encoding Myc-tagged PYK2. Cells were fixed 24 hours after transfection and immunostained with the polyclonal antibodies against PYK2 (amino acids 587-988) (A,C,E) or monoclonal antibodies against vinculin (B,D,F).





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