Fig. 6. Suppression of PYK2-induced cytoskeletal reorganization by FAK. Plasmids
encoding GFP (50 ng), FAK (25 ng, 50 ng and 100 ng), FAK-Y397F (50 ng) or FRNK
(50 ng) were co-injected with Myc-tagged PYK2 (50 ng) into Swiss 3T3 cells.
(A) Immunostaining of injected cells with antibodies against PYK2 (monoclonal,
Transduction Labs), FAK (BC3) or FRNK (BC3). (B) Histograms summarizing
results from the co-injection experiments. Microinjected cells with normal
focal adhesion or cell shape are shown as percentages of means ± s.d.
of three or more different samples (total of 200 microinjected cells).
PYK2+FAK (1:0.5), (1:1) and (1:2) represent ratios of plasmid DNA between PYK2
and FAK, which are 50:25 ng, 50:50 ng and 50:100 ng, respectively. Asterisk
represents a P value of <0.05 and double asterisks a P
value of <0.01 compared with the expression of PYK2 alone
(Mann-Whitney u test).
