Fig. 2. XMAP215 is a monomer. (A) Immunoblot of purified XMAP215 and a Xenopus egg
extract probed with an antibody raised against the C terminus of TOGp. The
antibody recognizes purified XMAP215 and a 215 kDa protein in Xenopus
egg extracts. Positions of Mr markers are shown. (B)
XMAP215 was incubated with (top row) or without (bottom row) an antibody to
its C terminus before uni-directional shadowing. In the presence of antibody,
the XMAP215 rod was decorated by a single globular structure that had the size
of an antibody molecule, suggesting that XMAP215 is a monomer. Scale bar: 100
nm.
