Fig. 3. EGF-dependent MHC-A localization in TSU-pr1 cells. Cells were grown on
cover slips coated with collagen I, stimulated with EGF and samples taken at
different time points, fixed, and prepared for indirect immunofluorescence
using the MHC-A affinity-purified antibodies as described in Materials and
Methods. In unstimulated TSU-pr1 cells (0 minutes), MHC-A was localized mainly
in the cytoplasm. 1 minute after EGF stimulation, most of the MHC-A
translocated to the cell cortex. The amount of MHC-A increased in the cortex
with the time of EGF stimulation (2-10 minutes). Bar, 10 µm.
