Fig. 2. Reverse phase HPLC analysis of radioactive products in islet cells. Cells
were pulse-labelled and then chased for 150 minutes under basal conditions
before stimulation with a cocktail of secretagogues for 60 minutes. The
representative profile is for radioactive products released during this 60
minute period with elution times for the two nonallelic rat I and II
proinsulins (PI) and their conversion products as indicated by the arrows.
*Elution times of the conversion intermediates des-31,32-split
proinsulin I and II, and des-64,65-split proinsulin I and II (reading from
left to right); tCP, truncated (des-(27-31)-) C-peptide.
