Fig. 5. Expression of Hub does not affect the release of proinsulin/insulin. Cells
were infected with adenovirus, sorted and labelled as described in the legend
to Fig. 3. Samples of medium,
following a 150 minute incubation under basal conditions and a subsequent 60
minute period of stimulation, as well as cell extracts (after the stimulation)
were analysed by HPLC (see Fig.
2). Labelled proinsulin and insulin (corrected for the difference
in the number of leucines in each) is presented as a percentage of that found
in all samples (basal + stimulated medium + extracts). Data are
mean±s.e.m., n=4.
