Fig. 6. Expression of Hub increases truncation of C-peptide. Cells were infected with adenovirus, sorted and labelled as described in the legend to Fig. 3. Radioactive truncated (des-(27-31)-) and intact C-peptide were quantified by HPLC (see Fig. 2). Truncated C-peptide is expressed as a percentage of total detectable labelled C-peptide (intact + truncated). Data are mean±s.e.m., n=4. ^*P<0.01 for Hub vs control for products released during the 150 minute basal chase.

Return to article