Fig. 4. ZW10 and ROD co-immunoprecipitate from Drosophila embryo and HeLa cell extracts. (A) Drosophila embryo extracts. Western blot probed with antibodies against DmZW10 shows the presence or absence of DmZW10 in immunoprecipitations from Drosophila embryo extracts. Immunoprecipitations used anti-DmZW10 crude (lane 2) or purified sera (lane 3), pre-immune serum from the DmZW10 injected rabbit (lane 1), anti-DmROD crude serum (lane 5), or pre-immune serum from the DmROD injected rabbit (lane 4). (B) HeLa cell extracts. Western blots were probed with antibodies against HsZW10. Lane 1 is crude HeLa cell extract. Other lanes on the blot show immunoprecipitations from the same HeLa cell extract: lane 3, anti-HsZW10 antibody; lane 2, pre-immune serum from the same rabbit; lane 5, anti-HsROD IgY; lane 4, pre-immune IgY from the same chicken. All samples are from the same western blot probed with the same anti-HsZW10 antibody, but the film exposure containing lanes 4 and 5 was for a longer period.
