Fig. 2. Immunofluorescence analysis of wild-type and chimeric syntaxins. PC12h cells were electroporated with the indicated HA-tagged wild-type syntaxins (a,c,e,g,i) or HA-tagged syntaxin 1A chimeras containing the transmembrane domain of syntaxin 5, 6, 7 or 8 (b,d,f,h, respectively), as described Materials and Methods. After incubation for 24 hours, the cells were treated for an additional 24 hours with 20 mM sodium butyrate. The cells were fixed and stained with anti-HA antibody. Syn1-5 localized to the CGN, as did wild-type syntaxin 5. Other chimeras were localized at the plasma membrane, but did not localize to the same compartments as did their wild-type counterparts containing the same transmembrane domains.
