Fig. 3. Time-lapse TIRF images of the elongation of individual integrin clusters at FCs. (A) Time-lapse TIRF images of integrin clustering for 15 minutes of recording. The left-hand column shows a series of TIRF images of an elongating integrin cluster in the central region of the cell. The right-hand column shows the corresponding fluorescence intensity profiles (arbitrary unit; a.u.) along the elongation path. Arrows show the tip of the integrin cluster, extending to the right. (B) A series of TIRF images of an elongating integrin cluster at the FC near the marginal region of the cell. Arrows point to the tip of an integrin cluster. The right-hand column indicates the corresponding fluorescence intensity profiles of the integrin cluster, showing that the extending cluster is brighter near the tip (arrowheads) and grows faster than those at the central region of the cell.
