Fig. 5. Simultaneous time-lapse imaging of integrin clusters at FCs by TIRF and CLSF microscopy. (A) A TIRF image of integrin clusters at 90 minutes after plating. The area surrounded by a rectangle was magnified and their dynamics were imaged by time-lapse TIRF and CLSF microscopy for 15 minutes as shown in B. Elongation of an integrin cluster at an FC was observed in the upper series of TIRF images (an arrow in each frame shows the tip of an extending integrin cluster), and a punctate spot of integrins in the intracellular space (an arrow in the lower series) was located slightly (
1 µm) above the tip of the integrin cluster at the FC. Bars, 10 µm (A) and 5 µm (B).
