Fig. 6. Time-lapse imaging of exocytosis and elongation of FCs. HUVECs were double stained with FM4-64, a marker for transport vesicles, and fluorescent anti-ß1-integrin antibody for 30 minutes before plating. (A) EpiF images observed at 60 minutes (a,b) and 90 minutes (c,d) after plating; (a,c) fluorescence spots from vesicles; (b,d) fluorescence spots from integrins. Arrowheads in a and b indicate the spots positive for both vesicles and integrins. The number of fluorescence spots decreased gradually, suggesting the progress of exocytosis (c). (B) TIRF images were observed 60 minutes (a,b) and 63 minutes (c,d) after plating. (a,c) The disappearance of fluorescence spots from vesicles (arrows). (b,d) The fluorescent spot from integrins and the spread of the bright integrin cluster. (C) TIRF images of the elongation of FCs. Images were taken at 60 minutes (a) and 75 minutes after plating (b). Red dots in b show the location of vesicle disappearance (putative exocytosis) during 15 minutes of observation.
