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Fig. 3. The ypt7 suppressors restore wild-type morphology of vacuoles. Abnormal vacuolar morphology of ccz1{Delta}/ccz1{Delta} cells (B), compared with wild-type CCZ1/CCZ1 (A) and suppressed cells ccz1{Delta}/ccz1{Delta} YPT7/ypt7K127E (C). Vacuoles were labelled with ade2 endogenous fluorophore. Cells were viewed by Nomarski optics (left) and the same fields were viewed for fluorescence (right). Wild-type vacuoles appear as large fluorescent spots corresponding to circular indentation in Nomarski. Vacuoles of mutant cells appear as numerous, fluorescent spots corresponding to irregular structures in Nomarski.





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