Fig. 2. Reporter construct and recombination analysis by PCR. (A) Map of the wild-type (WT) and R26R alleles, and the recombined R26R allele (Soriano, 1999). The LacZ gene was expressed upon Cre-mediated recombination. Primers used for PCR detection of genotype were 1/3 (WT), 1/2 (R26R), detection of recombination 1/Z3 (600 bp fragment) and detection of LacZ Z1/Z2 (Araki et al., 1995). (B) PCR analysis for Cre-mediated recombination of the R26R allele in (1) mammary gland epithelium in culture (P): -AdCre1: panel I, lanes 7,8, and panel II, lanes 1,10; +AdCre1: panel I, lanes 9,10 and panel II, lanes 2,9; (2) mammary epithelium outgrowth (O): -AdCre1: panel I, lanes 3,4 and panel II, lanes 5,6; +AdCre1: panel I, lanes 1,2 and panel II, lanes 3,4,7,8; (3) WT mouse tail DNA (M-): panel I, lane 11 and panel II, lane 11; (4) non-DNA control (-DNA): panel I, lane 12 and panel II, lane 12; (5) marker, 50 bp DNA ladder: panel I, lane 13; (6) WT mammary gland DNA (#3C): panel I, lanes 5,6.
