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Fig. 3. Immunolocalization of {alpha}T-catenin in cryosections of human heart. (A) Double immunofluorescent staining of {alpha}T-catenin (monoclonal antibody 892_24D2S) and {alpha}E-catenin (polyclonal antibody) shows colocalization of the {alpha}-catenin proteins at intercalated discs of cardiomyocytes. (B) Double immunofluorescent staining of {alpha}T-catenin (polyclonal antibody #952) and N-cadherin (monoclonal antibody) shows colocalization at intercalated discs of cardiomyocytes. (C) Immunohistochemical staining for {alpha}T-catenin (monoclonal antibody 892_24D2S) or desmin (monoclonal antibody 33) shows that {alpha}T-catenin is localized at the intercalated discs of cardiomyocytes, while desmin is present also at Z-discs. In the negative control (neg), only secondary antibody was used.





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